Image formation in three-photon fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
Three-photon excitation in fluorescence microscopy.
We show experiments proving the feasibility of scanning fluorescence microscopy by three-photon excitation. Three-photon excitation fluorescence axial images are shown of polystyrene beads stained with the fluorophore 2,5-bis(4-biphenyl)oxazole (BBO). Three-photon excitation is performed at an excitation wavelength of 900 nm and with pulses of 130 fs duration provided by a mode-locked titanium-...
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The invention of two-photon fluorescence light microscopy by Denk, Webb and co-workers (Denk et al., 1990) revolutionized three-dimensional (3D) in vivo imaging of cells and tissues. In 1931, the theoretical basis of twophoton excitation was established by Maria GöppertMayer, and this photophysical effect was verified experimentally by Kaiser and Garret in 1963. Two-photon excitation is a fluor...
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ژورنال
عنوان ژورنال: Bioimaging
سال: 1996
ISSN: 0966-9051,1361-6374
DOI: 10.1002/1361-6374(199609)4:3<124::aid-bio2>3.0.co;2-2